ACOUSTIC FILTRATION FOR CELL CULTURE

CYTOPERF

An acoustic filtration device for gentle cell manipulation and separation and remove the risk of clogging and fouling. Available in sterilizable or Single-use version

CYTOPERF - Accoustic Filter
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Acoustic filtration

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Cell concentration

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Gentle cell process

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Single use version available

Acoustic filtration for cell culture

Based on resonant ultrasonic waves that separate cells from supernatant, the CYTOPERF offers all the benefits of other filtration technologies, but without their inherent problems and limitations.

No cleaning needed thanks to the single use version of the CYTOPERF, no shear stress generated while filtering, no clogging, easy to install in parallel when it is needed to filter a high volume, etc.

This new technology is what you need in the field of filtration.

Acoustic filtration - CYTOPERF - IPRATECH

Advantages and benefits of our acoustic filtration system

Based on resonant ultrasonic waves that separate cells from supernatant, the CYTOPERF offers all the benefits of other filtration technologies,
but without their inherent problems and limitations.

Disposable

When cleaning and validation becomes an issue, or flexibility needs are increasing cost of good and capital investment, the CYTOPERF is available in a single-use version. All material in contact with media and cells are FDA approved and/or USP class VI certified.

No shear stress

As opposed to traditional frontal and tangential flow filtration or centrifugation, the acoustic force does not induce mechanical shear stress or pressure change to the cells, this results in gentle conditions and no loss of viability in this step of the process.

Non fouling non Clogging

As opposed to traditional flow filtration the CYTOPERF does not have any mechanical barrier with defined pore size that will suffer from cells debris or small particle accumulation.

Retains viable cells preferably

The dead cells or cell debris are less attracted in the acoustic nodes than the viable cells. This results is the selection of viable cells over cell debis that go away with the supernatant.

Freedom to operate with any bioreactor

The CYTOPERF is proposed as a stand-alone model for a very large range of volume to filter. When flow rate becomes high, it is extremely simple to operate several devices in parallel.

Cost effective

The economic advantages of this entirely disposable device are evident, and favorably comparable to other technologies, whether they are disposable or not.

Our acoustic filter proven applications

Perfusion

The CYTOPERF is used in combination with the CYTOSYS to control the perfusion process and manage viable cell density (VCD) according to projected performance parameters. The process can be maintained as long as required, from a week up to nine months. This Acoustic Perfusion system is the most advantageous solution for perfusion cultures. he Acoustic Perfusion system is the most advantageous solution for perfusion cultures.

Clarification and washing

The CYTOPERF device is ideal for cell concentration and cell washing after a cell culture as an early step in downstream process.

Continuous cell banking

Based on resonant ultrasonic waves that separate cells from supernatant, the CYTOPERF offers all the benefits of other perfusion technologies, but without their inherent problems and limitations.

Single cells / aggregates separation

CYTOPERF can also be set for separating cell aggregates that will stay in the filtrate from single cells that will move with the supernatant.

How our acoustic filtration work

How our acoustic filtration works ?

An ultrasonic standing wave field is generated at minimum power level to allow best cell separation from supernatant. Ultrasonic forces drive the cells to antinode planes parallel to the upward medium flow. These planes are visible through the windows as vertical parallel lines.
On a regular basis, the acoustic field is shut off to allow cells to settle before being flushed back to the bioreactor where the mixing movement disperses them. Power auto-tuning avoids overheating issues, ensuring the best separation efficiency regardless of cell concentration.

Due to their higher acoustic contrast, the acoustic filter retains viable cells with higher efficiency than dead cells and cell debris. This results in a significantly higher removal rate for non-viable cells, which successfully limits the accumulation of non-viable biomass in the bioreactor.